A substrate channel in the nitrogenase MoFe protein
نویسندگان
چکیده
منابع مشابه
The 16 th Fe in the Nitrogenase MoFe – Protein
[**]The work was supported by NIH (grant GM45162 to D.C.R.), NSERC PDF award (to L.M.Z.), and Deutsche Forschungsgemeinschaft (grants Ei–520/7 to O.E., An–676/1 to S.L.A.A., and IRTG 1478). G.M. is a Marie Curie International Outgoing Fellow (European Commission, grant no. 252961). We thank staff at Beamline 12–2, Stanford Synchrotron Radiation Lightsource (SSRL) and staff at HXMA Beamline, Can...
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In the nitrogenase molybdenum-iron (MoFe) protein, we have identified five potential substrate access pathways from the protein surface to the FeMo-cofactor (the active site) or the P-cluster using experimental structures of Xe pressurized into MoFe protein crystals from Azotobacter vinelandii and Clostridium pasteurianum. Additionally, all published structures of the MoFe protein, including th...
متن کاملP-cluster maturation on nitrogenase MoFe protein.
Biosynthesis of nitrogenase P-cluster has attracted considerable attention because it is biologically important and chemically unprecedented. Previous studies suggest that P-cluster is formed from a precursor consisting of paired [4Fe-4S]-like clusters and that P-cluster is assembled stepwise on MoFe protein, i.e., one cluster is assembled before the other. Here, we specifically tackle the asse...
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MgADP- reacted with the nitrogenase molybdenum-iron (MoFe) protein of Klebsiella pneumoniae (Kp1) over a period of 2 h to yield a stable, catalytically active conjugate. The isolated protein exhibited a new, broad 31P NMR resonance at -1 p.p.m. lacking phosphorus J coupling. The adenine ring of [8-14C]ADP remained associated with the conjugate. A covalently bound nucleotide was identified as AM...
متن کاملNitrogenase MoFe protein from Clostridium pasteurianum at 1.08 Å resolution: comparison with the Azotobacter vinelandii MoFe protein
The X-ray crystal structure of the nitrogenase MoFe protein from Clostridium pasteurianum (Cp1) has been determined at 1.08 Å resolution by multiwavelength anomalous diffraction phasing. Cp1 and the ortholog from Azotobacter vinelandii (Av1) represent two distinct families of nitrogenases, differing primarily by a long insertion in the α-subunit and a deletion in the β-subunit of Cp1 relative t...
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ژورنال
عنوان ژورنال: JBIC Journal of Biological Inorganic Chemistry
سال: 2009
ISSN: 0949-8257,1432-1327
DOI: 10.1007/s00775-009-0544-2